In this work we present a cleaner production (CP) method was applied to the production process of Erythropoietin in the biotechnological field. CP production potentials were identified from rapid and complete evaluations made during plant processes. Process areas where improvements in the use of water resources, energy, waste generation and waste disposal could be made were determined. The proposed CP method enabled the increase of environmental innovations of the studied process and in addition it can be applied to other biotechnological institutions with similar characteristics to those of the studied process.