The expansion of the use of biofuels is based on the implementation of public policies to support production, technological development and the market. In Brazil, the National Program for the Production and Use of Biodiesel (PNPB) seeks to articulate these incentives and attract private initiative in promoting sustainable production. Investigating the business actions for the development of new technologies in the face of public stimulation motivates this article that aims to analyze the Research and Development (R&D) strategies of Petrobras Biocombustível in the production of biodiesel. The case study is based on the theoretical arguments of the Entrepreneur State and on a case study accompanied by a qualitative analysis. The results indicate that the formation of agricultural research networks favored the construction of knowledge, research infrastructures and training of people, but did not innovated the production of biodiesel.