Several theories have been developed over a large number of years on the effort of localisation and exploitation of research results that take place in research laboratories and which can lead to the production of innovative products. This process is being facilitated by spin-off companies. The purpose of this paper is to shed light on this process of developing a spin-off company. To this goal a framework has been developed, namely “the Spin-off Chain”, which will be used as a guide and will provide to future entrepreneurs a roadmap exploiting the knowledge produced in academic and research laboratories.