Agave potatorum Zucc adventitious shoots were obtained in vitro from stem tissues and rooted in different solid media, pH 5.8, containing the following: 1) different concentrations (50, 75, and 100%) of MS (Murashige and Skoog) mineral salts and 2) different quantities of indole-3-butyric acid (IBA) (0, 0.5, 1 mg L-1). The shoots in each medium were separated into two groups and incubated for 84 days under different environments, including a laboratory or greenhouse. The experimental design was completely randomized with a 3×3×2 factorial arrangement. Adventitious roots emerged after 10 to 14 days of incubation, depending on the condition of incubation. In both laboratory and greenhouse settings, after 28 days of incubation, 66.6 and 93.6% of shoots subjected to treatment medium with 75% inorganic salts, 0.5 mg L-1 IBA and medium with 50% inorganic salts, 1 mg L-1 IBA, respectively, had formed roots. On day 40, all shoots had roots. After the 84-day rooting stage, the plants grown from the shoots in the medium with 1 mg L-1 IBA were larger in terms of leaf width, stem diameter, and largest leaf base cross-section area, but similar in the number of roots relative to the plants obtained in the media without IBA. Plant size increased with increasing mineral salt concentrations in the medium. Micropropagated plants obtained from the cultures incubated in a greenhouse and laboratory had an 8.9 and 5.4 cm leaf length, 1.8 and 0.8 cm³ leaf volume, 1.8 and 0.5 cm³ stem diameter, and 6.6 and 5.2 roots, respectively.