Abstract Today, a micropropagation method for Agave angustifolia exists, but for the multiplication of propagules, more information is needed on the diverse components of the culture medium, cytokinins and auxins and their effect on organogenetic response. The objective of this study was to evaluate dosages of benzylaminopurine and indol-3-acetic acid (IAA) in a culture medium and their effect on the formation of new adventitious shoots (organogenesis). The explants used were clusters of shoots on a common base of A. angustifolia stem tissue. Clusters of three to four shoots were established in different variants of a Murashige and Skoog culture medium that had different concentrations of benzylaminopurine (BAP; 0.5, 1, 2, and 4 mg L−1) and IAA) (0.1, 0.3, and 1 mg L−1). After 16 wk, it was found that a higher proliferation of shoots was positively related to the concentration of growth regulators. Explants formed a greater quantity of shoots in a culture medium with an increase in BAP of up to 4 mg L−1 and in IAA of up to 1 mg L−1, and there was a synergistic effect with BAP to induce the formation of the greatest number of shoots.