| dc.creator | UZAL,F.A. | |
| dc.creator | SMITH,P. | |
| dc.creator | NIELSEN,K. | |
| dc.creator | LIN,M. | |
| dc.creator | KELLY,W.R. | |
| dc.date | 1999-01-01 | |
| dc.date.accessioned | 2019-09-10T12:39:14Z | |
| dc.date.available | 2019-09-10T12:39:14Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0301-732X1999000100015 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/105071 | |
| dc.description | A fluorescence polarization assay (FPA) was developed to detect Clostridium perfringens type D epsilon antitoxin in serum of goats. Purified epsilon toxin was labelled with fluorescein isothiocyanate and used as a tracer in the test. Seven goat sera with different known concentrations of epsilon antitoxin (as measured by a mouse neutralization test-MNT) were used as positive controls. Sera from eleven colostrum-deprived kids were used as negative controls. The inter-assay variation of ten measurements of the seven positive sera varied between 1.56% and 4.85%. The correlation between the FPA values and concentration of epsilon antitoxin (as measured by the MNT) was 0.90. This test appears to be a good alternative to the MNT without most of the inconveniences of the later | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Facultad de Ciencias Veterinarias, Universidad Austral de Chile | |
| dc.relation | 10.4067/S0301-732X1999000100015 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Archivos de medicina veterinaria v.31 n.1 1999 | |
| dc.subject | fluorescence polarization assay | |
| dc.subject | goats | |
| dc.subject | Clostridium perfringens | |
| dc.subject | epsilon toxin antibodies | |
| dc.title | A homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats | |
