Archeological evidence suggest that the cultural developments occurred in the highlands around lake Titicaca in the Central Andes, exerted influence on the cultural elaborations of the human groups that peopled the valley of Azapa, close to the city of Arica, and the Pacific coast of northern Chile. In this communication we show by means of a distance analysis, that a craniofacial differentiation accompanied the process of cultural evolution in the valley. The biological influence of Tiwanaku is partially reflected in craniofacial morphology, providing preliminary evidence that cultural changes were associated to intermittent gene flow from the highlands, specially during the Alto Ramírez and San Miguel phases