| dc.creator | LEIVA-SALCEDO,ELIAS | |
| dc.creator | PEREZ,VIVIANA | |
| dc.creator | ACUÑA-CASTILLO,CLAUDIO | |
| dc.creator | WALTER,ROBIN | |
| dc.creator | SIERRA,FELIPE | |
| dc.date | 2002-01-01 | |
| dc.date.accessioned | 2019-09-10T12:42:15Z | |
| dc.date.available | 2019-09-10T12:42:15Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200020 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/107005 | |
| dc.description | Serum levels of T-kininogen increase dramatically as rats approach the end of their lifespan. Stable expression of the protein in Balb/c 3T3 fibroblasts leads to a dramatic inhibition of cell proliferation, as well as inhibition of the ERK signaling pathway. T-kininogen is a potent inhibitor of cysteine proteinases, and we have described that the inhibition of ERK activity occurs, at least in part, via stabilization of the MAP kinase phosphatase, MKP-1. Since fibroblasts are not a physiological target of T-kininogen, we have now purified the protein from rat serum, and used it to assess the effect of T-kininogen on endothelial cells. Adding purified T-kininogen to EAhy 926 hybridoma cells resulted in inhibition of basal ERK activity levels, as estimated using appropriate anti-phospho ERK antibodies. Furthermore, exogenously added T-kininogen inhibited the activation of the ERK pathway induced by either bradykinin or T-kinin. We conclude that the age-related increase in hepatic T-kininogen gene expression and serum levels of the protein could have dramatic consequences on endothelial cell physiology, both under steady state conditions, and after activation by cell-specific stimuli. Our results are consistent with T-kininogen being an important modulator of the senescent phenotype in vivo | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Sociedad de Biología de Chile | |
| dc.relation | 10.4067/S0716-97602002000200020 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Biological Research v.35 n.2 2002 | |
| dc.subject | aging | |
| dc.subject | endothelial cells | |
| dc.subject | ERK pathway | |
| dc.subject | kininogen | |
| dc.subject | kinins | |
| dc.title | T-kininogen inhibits kinin-mediated activation of ERK in endothelial cells | |
