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dc.creatorul-Haq,Ikram
dc.creatorAli,Sikander
dc.creatorQadeer,M.A.
dc.creatorIqbal,Javed
dc.date2002-08-01
dc.date.accessioned2019-11-14T12:56:06Z
dc.date.available2019-11-14T12:56:06Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200007
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/117513
dc.descriptionCitric acid fermentation of cane-molasses by submerged fermentation in 15 L stirred fermentor (working volume 9 L) was carried out. A hyper mutant strain of Aspergillus niger GCMC-7 was used in the present study which was obtained from the culture collection of our own labs. Ferrocyanide treated molasses [K4Fe(CN)6 200 ppm] medium containing sugar 150 g/l was employed as the basal fermentation medium. Different cultural conditions such as incubation temperature (30ºC), initial pH (6.0), air supply (1.0 l-1l-1min), agitation intensity (200 rpm) and time profile (144 h after inoculation) were optimised for enhanced citric acid production. Maximum amount of anhydrous citric acid obtained during the course of study was 106.65 g/l, with a sugar consumption of 107 g/l. Final pH, ferrocyanide concentration and dry cell mass were 2.1, 60 ppm and 16.5 g/l, respectively.
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dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34582002000200007
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.5 n.2 2002
dc.titleCitric acid fermentation by mutant strain of Aspergillus niger GCMC-7using molasses based medium


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