dc.creator | ul-Haq,Ikram | |
dc.creator | Ali,Sikander | |
dc.creator | Qadeer,M.A. | |
dc.creator | Iqbal,Javed | |
dc.date | 2002-08-01 | |
dc.date.accessioned | 2020-02-17T15:30:19Z | |
dc.date.available | 2020-02-17T15:30:19Z | |
dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200007 | |
dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/129009 | |
dc.description | Citric acid fermentation of cane-molasses by submerged fermentation in 15 L stirred fermentor (working volume 9 L) was carried out. A hyper mutant strain of Aspergillus niger GCMC-7 was used in the present study which was obtained from the culture collection of our own labs. Ferrocyanide treated molasses [K4Fe(CN)6 200 ppm] medium containing sugar 150 g/l was employed as the basal fermentation medium. Different cultural conditions such as incubation temperature (30ºC), initial pH (6.0), air supply (1.0 l-1l-1min), agitation intensity (200 rpm) and time profile (144 h after inoculation) were optimised for enhanced citric acid production. Maximum amount of anhydrous citric acid obtained during the course of study was 106.65 g/l, with a sugar consumption of 107 g/l. Final pH, ferrocyanide concentration and dry cell mass were 2.1, 60 ppm and 16.5 g/l, respectively. | |
dc.format | text/html | |
dc.language | en | |
dc.publisher | Pontificia Universidad Católica de Valparaíso | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.source | Electronic Journal of Biotechnology v.5 n.2 2002 | |
dc.title | Citric acid fermentation by mutant strain of Aspergillus niger GCMC-7using molasses based medium | |