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Differential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditions

dc.contributorFONDECYT, Marine Genomics Europe Technology Platforms Bid no. 43, FONDAP 1501-0001 (CONICYT) to the Center for Advanced Studies in Ecology & Biodiversityen-US
dc.creatorContreras-Porcia, Loretto
dc.creatorLópez-Cristoffanini, Camilo
dc.creatorLovazzano, Carlos
dc.creatorFlores-Molina, María Rosa
dc.creatorThomas, Daniela
dc.creatorNúñez, Alejandra
dc.creatorFierro, Camila
dc.creatorGuajardo, Eduardo
dc.creatorCorrea, Juan A.
dc.creatorKube, Michael
dc.creatorReinhardt, Richard
dc.date2017-03-07
dc.date.accessioned2020-11-05T13:35:15Z
dc.date.available2020-11-05T13:35:15Z
dc.identifierhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol41-issue5-fulltext-13
dc.identifier10.3856/vol41-issue5-fulltext-13
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/158485
dc.descriptionIn rocky shores, desiccation is triggered by daily tide changes, and experimental evidence suggests that local distribution of algal species across the intertidal rocky zone is related to their capacity to tolerate desiccation. In this context, the permanence of Pyropia columbina in the high intertidal rocky zone is explained by its exceptional physiological tolerance to desiccation. This study explored the metabolic pathways involved in tolerance to desiccation in the Chilean P. columbina, by characterizing its transcriptome under contrasting conditions of hydration. We obtained 1,410 ESTs from two subtracted cDNA libraries in naturally hydrated and desiccated fronds. Results indicate that transcriptome from both libraries contain transcripts from diverse metabolic pathways related to tolerance. Among the transcripts differentially expressed, 15% appears involved in protein synthesis, processing and degradation, 14.4% are related to photosynthesis and chloroplast, 13.1% to respiration and mitochondrial function (NADH dehydrogenase and cytochrome c oxidase proteins), 10.6% to cell wall metabolism, and 7.5% are involved in antioxidant activity, chaperone and defense factors (catalase, thioredoxin, heat shock proteins, cytochrome P450). Both libraries highlight the presence of genes/proteins never described before in algae. This information provides the first molecular work regarding desiccation tolerance in P. columbina, and helps, to some extent, explaining the classical patterns of ecological distribution described for algae across the intertidal zone.en-US
dc.formatapplication/pdf
dc.languageeng
dc.publisherPontificia Universidad Católica de Valparaísoen-US
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol41-issue5-fulltext-13/342
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol41-issue5-fulltext-13/148
dc.sourceLatin American Journal of Aquatic Research; Vol 41, No 5 (2013); 933-958en-US
dc.sourcePlataforma para envío de artículos - Latin American Journal of Aquatic Research; Vol 41, No 5 (2013); 933-958es-ES
dc.source0718-560X
dc.source0718-560X
dc.subjectPyropia; desiccation stress; ESTs; seaweeds; transcriptomics; proteinsen-US
dc.titleDifferential gene expression in Pyropia columbina (Bangiales, Rhodophyta) under natural hydration and desiccation conditionsen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeen-US
dc.typees-ES


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