Molecular characterization of infectious myonecrosis virus (IMNV) isolated from the shrimp Litopenaeus vannamei farmed in Ceará State, Brazil
Author
Coelho-Mello, Maria Verônyca
Florindo-Guedes, Maria Izabel
Rodriguez-Málaga, Sergio
de Almeida, Lia Magalhães
de Freitas Moreira, Mariana
de Oliveira, Tatiane Rodrigues
Full text
http://lajar.ucv.cl/index.php/rlajar/article/view/vol42-issue3-fulltext-2210.3856/vol42-issue3-fulltext-22
Abstract
The shrimp Litopenaeus vannamei, one of the most important species in world aquaculture, has seriously affected by infectious myonecrosis virus (IMNV) that causes up to 70% mortalities. With the aim of improving the development of new strategies for rapid and reliable diagnosis, we isolated IMNV, from L. vannamei farmed in Brazil, through a discontinuous sucrose gradient, and sequenced cDNA fragment encoding the major capsid protein from this virus. Nucleotides sequences corresponding to the viral capsid protein was obtained by RT-PCR and confirmed by automatic sequencing. Comparison with sequences which encode the capsid protein obtained from Indonesia isolates showed a high identity.