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Oxidative stress and RNA/DNA ratio following longline capture in the silky shark Carcharhinus falciformis (Müller & Henle, 1839)

dc.contributoren-US
dc.contributores-ES
dc.creatorCruz-Ramírez, Angélica
dc.creatorLiñan-Cabello, Marco Agustín
dc.creatorTavares, Rafael
dc.creatorSantana-Hernandez, Heriberto
dc.creatorPérez-Morales, Alfredo
dc.date2017-10-23
dc.date.accessioned2020-11-05T13:36:09Z
dc.date.available2020-11-05T13:36:09Z
dc.identifierhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol45-issue4-fulltext-23
dc.identifier10.3856/vol45-issue4-fulltext-23
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/158968
dc.descriptionThis study used transcriptomics tracers and measurements of oxidative stress to examine the effect of capture by longline on the silky shark Carcharhinus falciformis. From February 2010 to May 2012, C. falciformis individuals captured in the eastern Pacific Ocean were initially examined for sex, degree of maturity, and morphometric data. Samples were then taken from muscle tissue to determine RNA, DNA, and protein concentrations as well as RNA/DNA and protein/DNA ratios. The levels of three oxidative stress indicators [superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA)] were determined in muscle. The analysis of the 159 specimens (93 juveniles and 66 adults) showed low RNA/DNA ratios at the different stages of maturity. This basal level of transcriptomics capacity may have been caused by the physiological stress resulting from capture. In juveniles, the low RNA/DNA and protein/DNA ratios were associated with low levels of cellular damage related to oxidative stress, whereas in adults the level of cell damage due to oxidative stress was high, especially in older females. This pattern indicated decreased antioxidant response capacity with increasing age in elasmobranchsen-US
dc.descriptiones-ES
dc.formatapplication/pdf
dc.languageeng
dc.publisherPontificia Universidad Católica de Valparaísoen-US
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol45-issue4-fulltext-23/857
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol45-issue4-fulltext-23/926
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol45-issue4-fulltext-23/927
dc.sourceLatin American Journal of Aquatic Research; Vol 45, No 4 (2017); 846-851en-US
dc.sourcePlataforma para envío de artículos - Latin American Journal of Aquatic Research; Vol 45, No 4 (2017); 846-851es-ES
dc.source0718-560X
dc.source0718-560X
dc.subjectCarcharhinus falciformis; reactive oxygen species; RNA/DNA ratio; protein/DNA ratio; superoxide dismutase; malondialdehydeen-US
dc.subjectes-ES
dc.titleOxidative stress and RNA/DNA ratio following longline capture in the silky shark Carcharhinus falciformis (Müller & Henle, 1839)en-US
dc.titlees-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeen-US
dc.typees-ES


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