dc.contributor | Universidad de las Fuerzas Armadas - ESPE | en-US |
dc.creator | Tirado, Juan Ortiz | |
dc.creator | Valladares, Luis | |
dc.creator | Muñoz, Daysi | |
dc.creator | Caza, Jairo | |
dc.creator | Manjunatha, Bangeppagari | |
dc.creator | Kundapur, Rajesh R. | |
dc.date | 2018-03-15 | |
dc.date.accessioned | 2020-11-05T13:36:10Z | |
dc.date.available | 2020-11-05T13:36:10Z | |
dc.identifier | http://lajar.ucv.cl/index.php/rlajar/article/view/vol45-issue5-fulltext-8 | |
dc.identifier | 10.3856/vol45-issue5-fulltext-8 | |
dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/158979 | |
dc.description | Circulating levels of estradiol, vitellogenin and prostaglandins could be references of oocytes quality in the aquaculture industry. This study evaluated the performance of 17β-estradiol (E2) and vitellogenin (Vtg) levels in plasma and prostaglandins in oocytes (PGE 2) during the reproductive period of Oreochromis niloticus in a commercial farm in Ecuador. Adults fish females were treated with estradiol-synthetic (ES) and tilapia pituitary extracts (TP) during 25 days. For quantification of E2 and PGE 2, an enzyme-linked immunosorbent assay (ELISA) was used, while for Vtg relative quantification a Western blot analysis was performed. Circulating E2 concentrations were different during the first week of follow-up (P < 0.05) resulting in different levels of Vtg per treatment (P < 0.05) affecting on gonadal growth. The ovaries had an asynchronous development, where the mature oocytes proportions were higher with ES and TP than control. Relative fecundity was different between treatments (P < 0.05), where TP generated 15.7 ± 5.8 oocytes per g of spawning female. Furthermore, concentrations of PGE 2 were different between treatments (P < 0.05). The information reported in this study will be useful to improve the reproduction methods and have some biological signals before ovulation in tilapia commercial culture. | en-US |
dc.format | application/pdf | |
dc.language | eng | |
dc.publisher | Pontificia Universidad Católica de Valparaíso | en-US |
dc.relation | http://lajar.ucv.cl/index.php/rlajar/article/view/vol45-issue5-fulltext-8/870 | |
dc.source | Latin American Journal of Aquatic Research; Vol 45, No 5 (2017); 930-936 | en-US |
dc.source | Plataforma para envío de artículos - Latin American Journal of Aquatic Research; Vol 45, No 5 (2017); 930-936 | es-ES |
dc.source | 0718-560X | |
dc.source | 0718-560X | |
dc.subject | Oreochromis niloticus; tilapia; estradiol; vitellogenin; pituitary; prostaglandin E2 | en-US |
dc.title | Levels of 17β-estradiol, vitellogenin, and prostaglandins during the reproductive cycle of Oreochromis niloticus | en-US |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.type | | en-US |
dc.type | | es-ES |