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dc.contributorUniversidad de las Fuerzas Armadas - ESPEen-US
dc.creatorTirado, Juan Ortiz
dc.creatorValladares, Luis
dc.creatorMuñoz, Daysi
dc.creatorCaza, Jairo
dc.creatorManjunatha, Bangeppagari
dc.creatorKundapur, Rajesh R.
dc.date2018-03-15
dc.date.accessioned2020-11-05T13:36:10Z
dc.date.available2020-11-05T13:36:10Z
dc.identifierhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol45-issue5-fulltext-8
dc.identifier10.3856/vol45-issue5-fulltext-8
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/158979
dc.descriptionCirculating levels of estradiol, vitellogenin and prostaglandins could be references of oocytes quality in the aquaculture industry. This study evaluated the performance of 17β-estradiol (E2) and vitellogenin (Vtg) levels in plasma and prostaglandins in oocytes (PGE 2) during the reproductive period of Oreochromis niloticus in a commercial farm in Ecuador. Adults fish females were treated with estradiol-synthetic (ES) and tilapia pituitary extracts (TP) during 25 days. For quantification of E2 and PGE 2, an enzyme-linked immunosorbent assay (ELISA) was used, while for Vtg relative quantification a Western blot analysis was performed. Circulating E2 concentrations were different during the first week of follow-up (P < 0.05) resulting in different levels of Vtg per treatment (P < 0.05) affecting on gonadal growth. The ovaries had an asynchronous development, where the mature oocytes proportions were higher with ES and TP than control. Relative fecundity was different between treatments (P < 0.05), where TP generated 15.7 ± 5.8 oocytes per g of spawning female. Furthermore, concentrations of PGE 2 were different between treatments (P < 0.05). The information reported in this study will be useful to improve the reproduction methods and have some biological signals before ovulation in tilapia commercial culture.en-US
dc.formatapplication/pdf
dc.languageeng
dc.publisherPontificia Universidad Católica de Valparaísoen-US
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol45-issue5-fulltext-8/870
dc.sourceLatin American Journal of Aquatic Research; Vol 45, No 5 (2017); 930-936en-US
dc.sourcePlataforma para envío de artículos - Latin American Journal of Aquatic Research; Vol 45, No 5 (2017); 930-936es-ES
dc.source0718-560X
dc.source0718-560X
dc.subjectOreochromis niloticus; tilapia; estradiol; vitellogenin; pituitary; prostaglandin E2en-US
dc.titleLevels of 17β-estradiol, vitellogenin, and prostaglandins during the reproductive cycle of Oreochromis niloticusen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeen-US
dc.typees-ES


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