Partial characterization of digestive proteases in adults of bigclaw river shrimp Macrobrachium carcinus

Manríquez-Santos, Teresa de Jesús; Álvarez-González, Carlos Alfonso; Peña, Emyr; Camarillo-Coop, Susana; Martínez-García, Rafael; Vega-Villasante, Fernando

dc.contributor		es-ES
dc.contributor		en-US
dc.creator	Manríquez-Santos, Teresa de Jesús
dc.creator	Álvarez-González, Carlos Alfonso
dc.creator	Peña, Emyr
dc.creator	Camarillo-Coop, Susana
dc.creator	Martínez-García, Rafael
dc.creator	Vega-Villasante, Fernando
dc.date	2018-07-25
dc.date.accessioned	2020-11-05T13:36:19Z
dc.date.available	2020-11-05T13:36:19Z
dc.identifier	http://lajar.ucv.cl/index.php/rlajar/article/view/vol46-issue3-fulltext-5
dc.identifier	10.3856/vol46-issue3-fulltext-5
dc.identifier.uri	https://revistaschilenas.uchile.cl/handle/2250/159051
dc.description		es-ES
dc.description	The present research was focused to characterize the digestive proteases in Macrobrachium carcinus adults using biochemical and electrophoretic techniques. Our results showed that the alkaline proteolytic activity from males and females did not show significant differences (P > 0.05) between them, the optimum pH for digestive proteases is 8, and is very stable to changes in alkaline pH (8 and 10). The optimum temperature for alkaline proteases is 45ºC and is stable from 25 to 45ºC. The activity was totally inhibited with phenylmethylsulfonyl fluoride (PMSF), additionally the inhibition with trypsin soybean inhibitor 1 (SBT1) and tosyl-lysine-methyl ketone (TLCK) indicate high effect over serine proteases. Eight active bands were found using sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) zymogram (range 17.8-94.0 kDa), which were partially inhibited with ovalbumin (Ovo), SBT1, phenanthroline (Phen), tosyl-phenylalanine-methyl ketone (TPCK), TLCK and ethylenediaminetetraacetic acid (EDTA), indicating an omnivorous digestive capacity, which remarks that the mainly alkaline protease in M. carcinus hepatopancreas are trypsin like enzymes.	en-US
dc.format	application/pdf
dc.language	eng
dc.publisher	Pontificia Universidad Católica de Valparaíso	en-US
dc.relation	http://lajar.ucv.cl/index.php/rlajar/article/view/vol46-issue3-fulltext-5/945
dc.source	Latin American Journal of Aquatic Research; Vol 46, No 3 (2018); 525-533	en-US
dc.source	Plataforma para envío de artículos - Latin American Journal of Aquatic Research; Vol 46, No 3 (2018); 525-533	es-ES
dc.source	0718-560X
dc.source	0718-560X
dc.subject		es-ES
dc.subject	Macrobrachium carcinus; enzymes; nutrition; proteases; inhibitors	en-US
dc.title	Partial characterization of digestive proteases in adults of bigclaw river shrimp Macrobrachium carcinus	es-ES
dc.title	Partial characterization of digestive proteases in adults of bigclaw river shrimp Macrobrachium carcinus	en-US
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion
dc.type		en-US
dc.type		es-ES

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