The tiger shrimp Penaeus monodon is native to the Indo-West Pacific Ocean, covering the east coast of Africa, the Arabian Peninsula, Southeast Asia, Taiwan, China, the Sea of Japan, New Guinea and Australia. A new report was recorded on the capture in the wild of a female tiger shrimp P. monodon in conditions of reproductive maturity on the coast of Campeche in the Gulf of Mexico. The specimen was captured by a trawler operating near the coast of Campeche, in front of Carmen Island. The specimen was identified using dichotomous keys, additionally corroborated with analysis of the sequence of a fragment of 650 base pairs (bp) of the mitochondrial DNA (mtDNA) gene cytochrome oxidase I (COI), and compared with the sequence of P. monodon reported in GenBank. The specimen was deposited in the National Collection of Crustaceans of the Institute of Biology of the UNAM. Histological analysis of the gonads revealed that the organism was in a reproductive condition as ovaries contained oocytes in an advanced development state. The molecular data (sequenced fragments F and R) were identical with the COI sequence of P. monodon deposited in GenBank, thus confirming the presence of P. monodon from the coasts of Isla del Carmen, Campeche.