Dormitator latifrons is an amphidromous fish species distributed on the Pacific coastal region from California to Peru. It has a high potential to be cultured in Mexico. However, there is very little information about its biology, physiology and culture. This research study is a contribution to the hematology and blood chemistry of this native species. Results show hematocrit values of 28%, erythrocytes 2.075×106 mm3, leukocytes 35.035×103 mm3, mean corpuscular volume 161.547 fL, NBT 0.39, glucose 51.467 mg dL-1, protein 3.936 g dL-1, albumin 1.906 g dL-1, globulin 2.391 g dL-1 and albumin/globulin ratio 0.686.