Egg proteomic characterization of Seriola rivoliana in captivity
Author
Serrano-Pinto, Vania
Bernay, Benoit
Moreno-Legorreta, Manuel
Ortega-Rubio, Alfredo
Maldonado-García, Minerva
Full text
http://lajar.ucv.cl/index.php/rlajar/article/view/vol48-issue5-fulltext-238510.3856/vol48-issue5-fulltext-2385
Abstract
Continuous and sustained production of good quality eggs and larvae is required to have economically viable and ecologically sustainable rearing of Seriola rivoliana. Nonetheless, the complete production cycle of these species has been challenging to achieve due to high mortality during embryonic and larval stages. The objective of the project was to study the expression of the proteins involved in the embryonic process of almaco jack. Proteomic characterization of fertilized eggs was performed using two-dimensional electrophoresis and mass spectrometry. Different vitellogenin proteins A, B, C and Ab, β-actin, peroxiredoxin, superoxide dismutase 1, alpha subunit proteasome, and keratin II were identified to their functions related to embryonic development, energy metabolism, protein synthesis, cell structure, cytoskeleton, and antioxidant proteins with defense enzymatic activity.