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Isolation of actinomycetes from Nile tilapia (Oreochromis niloticus) cultivated in semi-intensive systems from Morelos, Mexico central zone

dc.contributorCONACyten-US
dc.contributorINIFAPen-US
dc.creatorParra-Laca, Rocio
dc.creatorHernández-Andrade, Laura
dc.creatorGarcía-Espinosa, Gary
dc.creatorLoza-Rubio, Elizabeth
dc.date2020-11-01
dc.date.accessioned2020-11-05T13:36:50Z
dc.date.available2020-11-05T13:36:50Z
dc.identifierhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol48-issue5-fulltext-2500
dc.identifier10.3856/vol48-issue5-fulltext-2500
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/159294
dc.descriptionThe production of Nile tilapia (Oreochromis niloticus) has good technological development; however, today, it is still necessary to make it more efficient. One way to increase efficiency is to prevent disease and improve the food conversion factor. Since previous investigations of tilapia microbiota detected a high proportion of organisms belonging to the order Actinomycetes, this study was to isolate, identify, and describe the species of bacteria microbiota belonging to the cultured Nile tilapia. These were done with Nile tilapia grown in a warm sub-humid climate during spring and summer seasons. The biopsy of different organs was performed for bacteriological culture and 16S rRNA sequencing analysis. From the 180 tissue samples, 49 isolates of the order Actinomycetes were obtained, representing ten species from seven genera: Microbacterium, Brevibacterium, Cellulomonas, Corynebacterium, Kocuria, Actinomyces, and Micrococcus. In spring, Microbacterium dominated, accounting for 74% of the total population. In the summer, lower diversity was observed, with 39% represented by Microbacterium. 16S rRNA sequencing analysis enabled the classification of Actinomyces neuii and Microbacterium lacticum as Kocuria varians and Agromyces indicus; the classification of Microbacterium imperiale as Rhodococcus and Micrococcus luteus was confirmed. No sequences of K. varians have been reported in fish. Microbacterium dextranoliticum showed high similarity to environmental samples. Here is the first study that analyzes the bacteria population in tilapia at the genetic level with an ecosystem approach, present in healthy cultured tilapia, indicating their beneficial associations with the host, making them candidates as probiotics, among other possible functions, applicable in tilapia cultivation.en-US
dc.formatapplication/pdf
dc.languageeng
dc.publisherPontificia Universidad Católica de Valparaísoen-US
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol48-issue5-fulltext-2500/1299
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol48-issue5-fulltext-2500/1904
dc.rightsCopyright (c) 2020 Latin American Journal of Aquatic Researchen-US
dc.sourceLatin American Journal of Aquatic Research; Vol 48, No 5 (2020); 758-767en-US
dc.sourcePlataforma para envío de artículos - Latin American Journal of Aquatic Research; Vol 48, No 5 (2020); 758-767es-ES
dc.source0718-560X
dc.source0718-560X
dc.subjectOreochromis niloticus; Nile tilapia; microbiota; Actinomycetes; seasonal variability; aquacultureen-US
dc.titleIsolation of actinomycetes from Nile tilapia (Oreochromis niloticus) cultivated in semi-intensive systems from Morelos, Mexico central zoneen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeen-US
dc.typees-ES


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