In this study, a range of physicochemical properties (dimensions, kernel volume, mass unit, bulk and true densities, porosity, terminal velocity, static friction coefficient against different surfaces, oil content and fatty acids composition) of the Bojnordi and Fakhri cultivars of grape seeds were studied. Fatty acid composition for both cultivars of grape seeds consisted of palmitic, stearic, oleic, linoleic and linolenic acid. The values for palmitic, stearic, oleic, linoleic and linolenic acid for the Bojnordi cultivar were 9.56, 4.37, 22.87, 62.85 and 0.33% and for the Fakhri cultivar were 9.22, 4.33, 19.75, 66.40 and 0.28%, respectively. All physical properties of the grape seed cultivars were significantly affacted by the moisture content in the studied range (11.5 to 23.9 w.b.%). For both cultivars (Bojnordi and Fakhri), the relationships between physical properties and moisture content were linear