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Microbial characterization of a natural biofilm associated with Peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysis

dc.contributorUniversidad Nacional de Tumbesen-US
dc.creatorGonzález-Pazmiño, José
dc.creatorPretell, Krizia Maribell
dc.creatorZapata-Vidaurre, Karina
dc.creatorMesones, Maribel Lucero
dc.creatorQuimí-Mujica, Juan
dc.creatorDiringer, Benoit
dc.date2021-03-01
dc.date.accessioned2021-03-19T15:07:23Z
dc.date.available2021-03-19T15:07:23Z
dc.identifierhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol49-issue1-fulltext-2547
dc.identifier10.3856/vol49-issue1-fulltext-2547
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/164931
dc.descriptionBiofilms strongly influence bivalve settlement patterns on artificial substrates; however, their structure and taxonomic composition remains a black box. We characterized a natural biofilm composition that exhibits a large settlement of larvae of the Peruvian scallop Argopecten purpuratus by culture-dependent and culture-independent methods. Thirty-two different strains, representing six genera (10 strains of Bacillus, 9 of Vibrio, 6 Acinetobacter, 4 Staphylococcus, 2 Photobacterium, and 1 Exiguobacterium) were isolated. Those strains represented only 1.09% of the relative abundance compared with the total microbiota obtained by 16S rRNA high-throughput sequencing. The metagenomic analysis identified 441 species. Prokaryotes were predominant (93.4%) over eukaryotes (6.6%), with Pelobacter (13.4%), Lewinella (5.6%), Marinobacter (5.4%), Hoeflea (4.2%), and Microcystis (3.1%) being the most representative genera. Laser scanning confocal microscopy (LSCM) imaging evidenced an irregular and heterogeneous biofilm with an average thickness of 35 μm, where the heterotrophic prokaryotic community (3.4×106 cell cm-2) dominate the photoautotrophic communities (2.3×105 cell cm-2). For the first time, an A. purpuratus settlement-related biofilm was described by the next generation sequencing tool (NGS) and compared with traditional methodologies.en-US
dc.formatapplication/pdf
dc.languageeng
dc.publisherPontificia Universidad Católica de Valparaísoen-US
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol49-issue1-fulltext-2547/1349
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol49-issue1-fulltext-2547/1959
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol49-issue1-fulltext-2547/1960
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol49-issue1-fulltext-2547/1961
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol49-issue1-fulltext-2547/1962
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol49-issue1-fulltext-2547/1963
dc.rightsCopyright (c) 2021 Latin American Journal of Aquatic Researchen-US
dc.sourceLatin American Journal of Aquatic Research; Vol 49, No 1 (2021); 86-96en-US
dc.sourcePlataforma para envío de artículos - Latin American Journal of Aquatic Research; Vol 49, No 1 (2021); 86-96es-ES
dc.source0718-560X
dc.source0718-560X
dc.subjectArgopecten purpuratus; biofilm; microbiota; settlement; larvae; NGS; LSCMen-US
dc.titleMicrobial characterization of a natural biofilm associated with Peruvian scallop (Argopecten purpuratus) larvae settlement on artificial collector by confocal imaging, microbiology, and metagenomic analysisen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeen-US
dc.typees-ES


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