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Partial characterization of digestive proteases in Pacific red snapper Lutjanus peru Nichols & Murphy, 1922 (Perciformes: Lutjanidae)

dc.contributoren-US
dc.creatorPeña-Marín, Emyr S.
dc.creatorIbarra-Castro, Leonardo
dc.creatorMartínez-Brown, Juan M.
dc.creatorHernández-López, Iris A.
dc.creatorTovar-Ramírez, Dariel
dc.creatorPérez-Urbiola, Juan C.
dc.creatorMorales-García, Vicente
dc.creatorMartinez-García, Rafael
dc.creatorCamarillo Coop, Susana
dc.creatorMartínez-Montaño, Emmanuel
dc.creatorÁlvarez-González, Carlos A.
dc.date2021-07-04
dc.date.accessioned2021-07-15T14:58:45Z
dc.date.available2021-07-15T14:58:45Z
dc.identifierhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol49-issue3-fulltext-2624
dc.identifier10.3856/vol49-issue3-fulltext-2624
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/170016
dc.descriptionPacific red snapper (Lutjanus peru) is an important commercial species in Mexico with great aquaculture potential; however, digestive physiology is still unknown. Therefore, the objective of the present work was to characterize the digestive proteases of L. peru juvenile using biochemical and electrophoretic techniques. Results showed a higher acid protease activity than the alkaline proteases, trypsin, chymotrypsin, and leucine aminopeptidase (LAP). The optimum temperature for acid proteases was between 30 to 40°C. Trypsin activity showed two maximum peaks of temperature (30 and 50°C), while alkaline proteases, chymotrypsin, and LAP had optimum temperatures of 50, 50 to 60, and 40°C, respectively. Moreover, the optimum pH of acid proteases was between 2 and 3. Also, alkaline proteases, trypsin, chymotrypsin showed pH optimums at pH 6, 9, and 5, respectively, although LAP showed two optimum pH values at 6 and 9. Acid protease zymogram showed three isoforms, totally inhibited by pepstatin A. Alkaline protease zymogram revealed six bands (125.4, 67.2, 57.9, 48.6, 29.8, and 26.9 kDa), which were inhibited by specific serine-proteases and metalloproteases inhibitors. In conclusion, the main digestion in L. peru depends on stomach proteases, which are characteristic of carnivorous fish, followed by intestinal digestion supported mainly by chymotrypsin.en-US
dc.formatapplication/pdf
dc.languageeng
dc.publisherPontificia Universidad Católica de Valparaísoen-US
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol49-issue3-fulltext-2624/1405
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol49-issue3-fulltext-2624/2035
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol49-issue3-fulltext-2624/2036
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol49-issue3-fulltext-2624/2037
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol49-issue3-fulltext-2624/2038
dc.rightsCopyright (c) 2021 Latin American Journal of Aquatic Researchen-US
dc.sourceLatin American Journal of Aquatic Research; Vol 49, No 3 (2021); 442-450en-US
dc.sourcePlataforma para envío de artículos - Latin American Journal of Aquatic Research; Vol 49, No 3 (2021); 442-450es-ES
dc.source0718-560X
dc.source0718-560X
dc.subjectLutjanus peru; proteases; enzymes; inhibitors; physiology; characterization; aquacultureen-US
dc.titlePartial characterization of digestive proteases in Pacific red snapper Lutjanus peru Nichols & Murphy, 1922 (Perciformes: Lutjanidae)en-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeen-US
dc.typees-ES


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