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Gut bacterial profile associated with healthy and diseased (AHPND) shrimp Penaeus vannamei

dc.contributoren-US
dc.creatorAfrin, Sarah
dc.creatorAlvarez-Ruiz, Píndaro
dc.creatorLuna-González, Antonio
dc.creatorEscamilla-Montes, Ruth
dc.creatorFierro-Coronado, Arturo
dc.creatorDiarte-Plata, Genaro
dc.creatorGarcía-Gutiérrez, Viridiana
dc.creatorPeraza-Gómez, Viridiana
dc.date2022-05-01
dc.identifierhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol50-issue2-fulltext-2839
dc.identifier10.3856/vol50-issue2-fulltext-2839
dc.descriptionThe effect of Vibrio parahaemolyticus IPNGS16 on the bacterial profile of the gut of Penaeus vannamei was assessed by 16S metagenomic analysis. The V3 hypervariable region of the bacterial 16S rDNA was amplified by PCR. Sequencing reads were generated using the 2×150 (300 cycles) for the base-read length chemistry of the Illumina MiniSeq platform. The web-based Shaman and MicrobiomeAnalyst platforms were used to analyze the sequences. The phyla Proteobacteria, Bacteroidetes, and the genera Vibrio, Ruegeria, Nautella, and Pseudoalteromonas were found among the most abundant taxonomic ranks in control, diseased, and healthy shrimp. Alpha and beta indices showed significant differences between shrimp survival in the control condition and dying shrimp (lower diversity). Metabolism (carbohydrate and amino acid metabolism-related genes and, to a lesser extent, energy, lipid, and cofactors and vitamin metabolism-related genes) of dying and surviving shrimp was affected by Vibrio infection. The top metabolic functions (cell cycle, glycine, serine, threonine, cysteine, methionine, purine, pyrimidine, pyruvate, and quorum sensing) in dying and surviving shrimp were affected by Vibrio, especially quorum sensing. The interaction network analysis showed fewer interactions in dying shrimp than control and surviving shrimp. Proteobacteria, Bacteroidetes, Vibrio, and Ruegeria predominated in all samples, and Vibrio changed bacterial diversity and metabolism in the intestine of P. vannamei. Ruegeria and Pseudoalteromonas showed negative interactions with Vibrio, suggesting their use as probiotics. This study sheds light on the Vibrio infection in the gut microbiota of shrimp.en-US
dc.formatapplication/pdf
dc.languageeng
dc.publisherPontificia Universidad Católica de Valparaísoen-US
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol50-issue2-fulltext-2839/1686
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol50-issue2-fulltext-2839/2227
dc.rightsCopyright (c) 2022 Latin American Journal of Aquatic Researchen-US
dc.sourceLatin American Journal of Aquatic Research; Vol 50, No 2 (2022); 197-211en-US
dc.sourcePlataforma para envío de artículos - Latin American Journal of Aquatic Research; Vol 50, No 2 (2022); 197-211es-ES
dc.source0718-560X
dc.source0718-560X
dc.subjectPenaeus vannamei; Vibrio parahaemolyticus; metabolism; metagenomics; microbiota; aquacultureen-US
dc.titleGut bacterial profile associated with healthy and diseased (AHPND) shrimp Penaeus vannameien-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeen-US
dc.typees-ES


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