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What lies beneath? Revealing biodiversity through eDNA analysis in Lobos de Afuera Islands, Peru

dc.contributorThe World Bank through the Global Environmental Facility (GEF) project at the Guano Islands, Isles and Capes National Reserve – GEF Guanerasen-US
dc.creatorAlfaro-Cordova, Eliana
dc.creatorOrtiz-Alvarez, Clara
dc.creatorAlfaro-Shigueto, Joanna
dc.creatorMangel, Jeffrey C.
dc.creatorGarcía, Oscar
dc.creatorVelez-Zuazo, Ximena
dc.date2022-08-31
dc.date.accessioned2022-09-26T20:21:02Z
dc.date.available2022-09-26T20:21:02Z
dc.identifierhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol50-issue4-fulltext-2782
dc.identifier10.3856/vol50-issue4-fulltext-2782
dc.identifier.urihttps://revistaschilenas.uchile.cl/handle/2250/209757
dc.descriptionEnvironmental DNA (eDNA) has become a powerful tool for assessing biodiversity in different environments and may be a complementary method compared to traditional methods to assess biodiversity. We tested eDNA as a complementary tool to assess marine biodiversity at Lobos de Afuera islands (ILA) in Peru. Nine water samples were collected from three sites within ILA using a commercial eDNA kit and then analyzed using vertebrate, teleost, and marine mammal primers targeting the 12S rRNA gene. Operational taxonomic units (OTUs) classified at order, family, genus, and species levels were compared to baseline reports obtained through visual survey methods. Compared with traditional methods, eDNA assays identified 26% fewer species. However, it was a cost-effective method due to the higher number of identified bony fish species per sampling unit. The eDNA assays provided a broader representation of higher taxonomic levels (order, family, and genus), with a higher sensitivity for bony fish than the traditional methods used. Also, the same numbers of orders and families reported by visual assessments were detected with eDNA. Our study shows practical implications for using eDNA for biota assessments in remote and isolated areas. Future efforts should aim to catalog the biodiversity from inaccessible places using eDNA-methods.en-US
dc.formatapplication/pdf
dc.languageeng
dc.publisherPontificia Universidad Católica de Valparaísoen-US
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/view/vol50-issue4-fulltext-2782/1611
dc.relationhttp://lajar.ucv.cl/index.php/rlajar/article/downloadSuppFile/vol50-issue4-fulltext-2782/2176
dc.rightsCopyright (c) 2022 Latin American Journal of Aquatic Researchen-US
dc.sourceLatin American Journal of Aquatic Research; Vol 50, No 4 (2022); 642-659en-US
dc.sourcePlataforma para envío de artículos - Latin American Journal of Aquatic Research; Vol 50, No 4 (2022); 642-659es-ES
dc.source0718-560X
dc.source0718-560X
dc.subjectenvironmental DNA; marine biodiversity; monitoring; marine vertebrates; Peruen-US
dc.titleWhat lies beneath? Revealing biodiversity through eDNA analysis in Lobos de Afuera Islands, Peruen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeen-US
dc.typees-ES


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