| dc.contributor | | en-US |
| dc.creator | Balderas-González, Verónica | |
| dc.creator | Ponce-Rivas, Elizabeth | |
| dc.creator | Díaz, Fernando | |
| dc.creator | Camacho-Jiménez, Laura | |
| dc.creator | Sánchez-Castrejón, Edna | |
| dc.creator | Aguilar, Manuel B. | |
| dc.date | 2023-04-30 | |
| dc.date.accessioned | 2023-05-09T15:57:03Z | |
| dc.date.available | 2023-05-09T15:57:03Z | |
| dc.identifier | http://lajar.ucv.cl/index.php/rlajar/article/view/vol51-issue2-fulltext-2967 | |
| dc.identifier | 10.3856/vol51-issue2-fulltext-2967 | |
| dc.identifier.uri | https://revistaschilenas.uchile.cl/handle/2250/225386 | |
| dc.description | The crustacean hyperglycemic hormone (CHH) is a multifunctional neuropeptide that plays a central role in crustacean metabolism and physiology. Experiments were conducted to examine the metabolic and osmoionic capabilities of the white shrimp Penaeus vannamei injected with its recombinant CHH-A (rCHH-A) peptide and acutely transferred from iso- (26 ppt) to hypo- (10 ppt) and hyper-osmotic (40 ppt) conditions. Hemolymph glucose, protein, osmoregulatory capacity (OC), and ionoregulatory capacity (IC) for sodium, chloride, potassium, and calcium were evaluated at four post-injection times (0.5, 1, 2, and 24 h). The rCHH-A peptide had hyperglycemic activity in all salinity conditions, obtaining maximum values at 1 h post-injection. However, in shrimp transferred to hyper-osmotic condition, rCHH-A caused the most significant reductions in OC (2 h), chloride IC (2 h), and total proteins (0.5-2 h) compared to the phosphate-buffered saline (PBS) control. Contrastingly, in shrimp transferred to hypo-osmotic conditions, rCHH-A decreased OC significantly from 2-24 h, strongly decreased chloride and potassium IC at 1 h post-injection, and increased total protein concentration in the hemolymph (1-2 h) when compared to PBS control. Concerning calcium, the rCHH-A injection decreased calcium IC at 10 ppt (1 h) and 26 ppt (2 h), providing insight into a potential role for CHH-A in calcium regulation. The results suggest that glucose and protein mobilization could enhance energy for osmo-ionic regulation under extreme osmotic conditions. This research study contributed to understanding crustacean endocrinology in P. vannamei and related euryhaline crustaceans. Further research should be performed to understand the osmo- and ionoregulatory mechanisms of the different CHH variants in crustaceans exposed to other stress conditions and the relationship with intermediary energy metabolism regulation. | en-US |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | en-US |
| dc.relation | http://lajar.ucv.cl/index.php/rlajar/article/view/vol51-issue2-fulltext-2967/1694 | |
| dc.rights | Copyright (c) 2023 Latin American Journal of Aquatic Research | en-US |
| dc.source | Latin American Journal of Aquatic Research; Vol 51, No 2 (2023); 248-267 | en-US |
| dc.source | Plataforma para envío de artículos - Latin American Journal of Aquatic Research; Vol 51, No 2 (2023); 248-267 | es-ES |
| dc.source | 0718-560X | |
| dc.source | 0718-560X | |
| dc.subject | Penaeus vannamei; crustacean hyperglycemic hormone; glucose; osmoregulatory capacity; ion concentrations; recombinant peptide | en-US |
| dc.title | Metabolic and osmoionic effects of the recombinant crustacean hyperglycemic hormone (rCHH-A) of the Pacific white shrimp Penaeus vannamei on specimens acutely exposed to extreme salinities | en-US |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:eu-repo/semantics/publishedVersion | |
| dc.type | | en-US |
| dc.type | | es-ES |
