In this paper, we investigate an inverse source problem involving a one-dimensional diffusion equation of a time-fractional RiemannLiouville derivative with 0 < α < 1. First, results on the existence and regularity of the weak solution of the direct problem are obtained. For the determination of the unknown time-dependent source term, we use a monotone and distinguishable input-output mapping defined by the additional over-determination integral data for the considered sub-diffusion problem. Finally, the uniqueness of the solution of the inverse problem is proved.