The effect of a partial substitute of fish meal by biofloc meal on the growth and survival of fry Nile tilapia Oreochromis niloticus was evaluated over nine weeks. Two biofloc meals were obtained by drying them in two processes: A) sun exposure and B) convection oven. A randomized design of eight isonitrogenous (40%) and isoenergetic (17 kJ g-1) diets was used, where 5, 10, 15, and 20% of the fish meal was substituted for the two biofloc meals. The nutritional study was conducted in a recirculating aquaculture system with Nile tilapia fry (initial weight: 0.64 ± 0.16 g). The survival range was similar in all treatments and higher than 97%. Tilapia fed with the treatments A5, A10, B5, and B10 of substitution of biofloc meal showed similar and significantly better final weight, individual weight gain, specific growth rate, and condition factor than A15, A20, B15, and B20 treatments; as well as efficiency of use of the feed: protein efficiency ratio, carcass nitrogen deposition, apparent nitrogen utilization, and apparent digestibility. Therefore, these results suggest that biofloc meal is possible in diets for tilapia, thus reducing the need for fishmeal as the main source of protein in the diet.