In recent years, Brazil has been facing the effects of COVID-19. The need for social isolation has affected 24-hour movement behaviors and the psychological well-being of children and adolescents. The main objective of this study is to evaluate possible associations between 24-hour movement behaviors and anxiety levels in preschool children after social isolation. The sample consisted of 61 children of both sexes, aged between three and six years, enrolled in public schools. Adapted questionnaires were applied to assess 24-hour movement behaviors and anxiety. We used descriptive analysis through frequency distribution and network analysis. It was observed that children do not adhere to physical activity recommendations during the week or on weekends. Being hurt and dependence on parents were the anxiety-related behaviors with the highest values. Adhering to physical activity recommendations during the week does not guarantee compliance with other behaviors. Anxious behavior tends to influence the increase of other behaviors. The variables most likely to generate changes in the network were: fear of separation, dependence on parents, symptoms of fear and anxiety, and screen use during the weekend. It can be concluded that not complying with 24-hour movement behavior recommendations can increase anxiety levels. It is suggested that more studies focused on this topic be developed, considering its high relevance.In recent years, Brazil has been facing the effects of COVID-19. The need for social isolation has affected 24-hour movement behaviors and the psychological well-being of children and adolescents. The main objective of this study is to evaluate possible associations between 24-hour movement behaviors and anxiety levels in preschool children after social isolation. The sample consisted of 61 children of both sexes, aged between three and six years, enrolled in public schools. Adapted questionnaires were applied to assess 24-hour movement behaviors and anxiety. We used descriptive analysis through frequency distribution and network analysis. It was observed that children do not adhere to physical activity recommendations during the week or on weekends. Being hurt and dependence on parents were the anxiety-related behaviors with the highest values. Adhering to physical activity recommendations during the week does not guarantee compliance with other behaviors. Anxious behavior tends to influence the increase of other behaviors. The variables most likely to generate changes in the network were: fear of separation, dependence on parents, symptoms of fear and anxiety, and screen use during the weekend. It can be concluded that not complying with 24-hour movement behavior recommendations can increase anxiety levels. It is suggested that more studies focused on this topic be developed, considering its high relevance.En los últimos años, Brasil ha enfrentado los efectos de la COVID-19. La necesidad de aislamiento social ha afectado las conductas de movimiento durante las 24 horas y el bienestar psicológico de niñas, niños y adolescentes. El principal objetivo del presente trabajo es evaluar posibles asociaciones entre las conductas de movimiento durante las 24 horas y los niveles de ansiedad en niñas y niños preescolares después del aislamiento social. La muestra estuvo compuesta por 61 niñas y niños de ambos sexos, con edades entre tres y seis años, matriculados en escuelas públicas. Se aplicaron cuestionarios adaptados para evaluar las conductas de movimiento y la ansiedad durante las 24 horas. Se utilizó un análisis descriptivo mediante la distribución de frecuencias y análisis de redes. Se observó que las niñas y los niños no siguen las recomendaciones de actividad física tanto durante la semana como el fin de semana. Sentirse herido y la dependencia de los progenitores fueron las conductas de ansiedad con valores más altos. Cumplir con las recomendaciones de actividad física durante la semana no garantiza el cumplimiento de otras conductas. Las conductas ansiosas tienden a influir en el aumento de las demás. Las variables con mayor probabilidad de generar cambios en la red fueron: miedo a la separación, dependencia de los progenitores, síntomas de miedo y ansiedad, y el uso de pantallas durante el fin de semana. Se concluye que no cumplir con las recomendaciones de las conductas de movimiento durante las 24 horas puede aumentar los niveles de ansiedad. Se sugiere que se desarrollen más estudios enfocados en este tema, considerando su alta pertinencia.Nos últimos anos, o Brasil vem enfrentando os efeitos da COVID-19. A necessidade de isolamento social afetou os comportamentos de movimento 24 horas e o bem-estar psicológico de crianças e adolescentes. O presente trabalho tem como principal objetivo avaliar possíveis associações entre os comportamentos de movimento 24 horas e os níveis de ansiedade em crianças pré-escolares após o isolamento social. A amostra foi composta por 61 crianças de ambos os sexos, com idades entre três e seis anos, matriculadas em escolas públicas. Foram aplicados questionários adaptados para avaliar os comportamentos de movimento 24 horas e ansiedade. Utilizou-se análise descritiva por meio da distribuição de frequência e análise de redes. Verificou-se que as crianças não aderem às recomendações de atividade física tanto durante a semana quanto no final de semana. Os comportamentos de ansiedade com valores mais altos foram "ficar magoado" e "dependência dos pais". Aderir às recomendações de atividade física durante a semana não garante o cumprimento dos demais comportamentos. Um comportamento ansioso tende a influenciar o aumento de outros. As variáveis mais propensas a gerar modificações na rede foram: medo de separação, dependência dos pais, sintomas de medo e ansiedade, e uso de telas durante o fim de semana. Conclui-se que não cumprir as recomendações dos comportamentos de movimento 24 horas pode aumentar os níveis de ansiedade. Sugere-se que mais estudos voltados para essa temática sejam desenvolvidos, considerando que é um tema de grande relevância.