This study describes the qualitative and quantitative seasonal analysis of the essential oils from an unexplored plant Eugenia brevistyla, native from Brazilian Atlantic Rain Forest and Semidecidual Forest. Analysis by GC-FID and GC-MS allowed the identification of 28 compounds. The largest fraction corresponds to oxygenated sesquiterpenes in all seasons. The major compound was E-nerolidol in all seasons, being higher in winter (83.14%) and lower in spring (69.6%). The second major compound was byciclogermacrene in the spring and in the summer essential oils. Alloaromadendrene and spathulenol were the second major compounds in autumn and winter, respectively. Sesquiterpenes hydrocarbons showed higher variation along the year (58%) than oxygenated sesquiterpenes (2%). No monoterpenes were found in the analyzed essential oils.