This paper presents the first inventory of ammonia emissions from livestock production in Los Lagos and Los Ríos Regions of Chile. The inventory was focused in this area because is where cattle production is concentrated. This study aimed to quantify the amount of N losses due to ammonia volatilization from livestock production in these regions of the country, and to provide its spatial representation identifying their main sources. Calculations have been carried out for 1997 and 2007, and also projections to 2017 and 2027 were obtained. Calculated emissions were 6097 and 6206 t NH3-N for 1997 and 2007, respectively. Major sources of NH3-N emissions were cattle accounting for more than 85%, followed by horses and pigs. Farm management practices as grazing, housing, manure storage and land spreading of manure accounted for 87%, 9%, 3%, and 2%, respectively, of total annual emissions. Projections suggest that emissions could increase up to 6344 and 6516 t NH3-N for 2017 and 2027, respectively. Emissions are much lower than those reported for developed European countries. However, intensification of cattle production systems may lead to an increase of ammonia emissions which could impact the temperate forest of Southern Chile characterized by low nutrient fluxes.