Show simple item record

Endogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) Oerst

dc.creatorCartes Riquelme,Pricila
dc.creatorRíos Leal,Darcy
dc.creatorSáez Carrillo,Kátia
dc.creatorUribe Moraga,Matilde
dc.creatorValenzuela Aguilar,Sofía
dc.creatorJoseph Bolus,Stephen
dc.creatorSánchez Olate,Manuel
dc.date2011-12-01
dc.date.accessioned2019-04-24T21:19:13Z
dc.date.available2019-04-24T21:19:13Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000400007
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/55645
dc.descriptionAbscisic acid (ABA) and indole-3-acetic acid (IAA) participate in the propagation of plants by somatic embryogenesis, causing polar structural differentiation of the embryo. The goal of the assay was to compare endogenous levels of ABA and IAA between somatic embryos (SE) and zygotic embryos (ZE) of Nothofagus alpina (Poepp. & Endl.) Oerst. In this study, a somatic embryo maturation assay involving the addition of varying concentrations of exogenous ABA was performed on cotyledonary-stage of N. alpina. Furthermore, the endogenous levels of ABA and IAA were quantified in the immature ZE, the mature ZE, and the embryonic axis of a mature embryo of N. alpina. The current study utilized high performance liquid chromatography (HPLC) for quantification. The maturation treatments performed did not present significant differences in the endogenous ABA levels in SE. However, significant differences did exist in levels of ABA and IAA between SE submitted to the different maturation treatments and mature ZE of N. alpina. The application of exogenous ABA to the culture medium increased endogenous ABA levels, therefore, increasing the number of germinated somatic embryos. Thus, the plant conversion process was also successfully completed in somatic embryos of N. alpina.
dc.formattext/html
dc.languageen
dc.publisherInstituto de Investigaciones Agropecuarias, INIA
dc.relation10.4067/S0718-58392011000400007
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceChilean journal of agricultural research v.71 n.4 2011
dc.subjectSomatic embryogenesis
dc.subjectmaturation
dc.subjectgermination
dc.subjectHPLC
dc.titleEndogenous Quantification of Abscisic Acid and Indole-3-Acetic Acid in Somatic and Zigotic Embryos of Nothofagus alpina (Poepp. & Endl.) Oerst


This item appears in the following Collection(s)

Show simple item record