| dc.creator | Lavakumaran,Lavanya | |
| dc.creator | Seran,Thayamini H | |
| dc.date | 2014-12-01 | |
| dc.date.accessioned | 2019-04-24T21:20:00Z | |
| dc.date.available | 2019-04-24T21:20:00Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-58392014000400018 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/55962 | |
| dc.description | Aloe (Aloe vera [L.] Burm. f.) is an important medicinal herb and is propagated vegetatively. However, its propagation rate is slow and cannot supply good quality planting material to large-scale growers. It is therefore necessary to use in vitro clonal propagation for commercial purposes. The aim of this study was to select a suitable medium for initial culture establishment and subsequent shoot multiplication of aloe. Shoot tips were excised from aloe plants and disinfected with 20% Clorox (5.25% sodium hypochlorite) for 30 min. Sterilized shoot tips were vertically dissected into quarters and then inoculated on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) or thidiazuron (TDZ) (1, 2, and 3 mg L-1) combined with 0.5 mg L-1 1-naphthaleneacetic acid (NAA). Results revealed that there was a significant difference (P < 0.05) between different hormone combinations in shoot bud formation. Explants placed in 3 mg L-1 BAP and 0.5 mg L-1 NAA produced significantly (P < 0.05) higher numbers of microshoots than other tested treatments. The medium containing a low concentration of TDZ (1 mg L-1) with NAA produced multiple microshoots, whereas the highest TDZ concentration (3 mg L-1) with NAA produced high levels of abnormalities. Transferring microshoot buds from 1 mg L-1 TDZ to 3 mg L-1 BAP accelerated production of prominent and elongated shoots. The MS medium supplemented with 3 mg L-1 BAP and 0.5 mg L-1 NAA produced higher number of normal shoots per explant of aloe under in vitro conditions. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Instituto de Investigaciones Agropecuarias, INIA | |
| dc.relation | 10.4067/S0718-58392014000400018 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Chilean journal of agricultural research v.74 n.4 2014 | |
| dc.subject | Aloe | |
| dc.subject | BAP | |
| dc.subject | microshoots | |
| dc.subject | in vitro culture | |
| dc.subject | organogenesis | |
| dc.subject | TDZ | |
| dc.title | Effect of 6-benzyl-aminopurine and thidiazuron on in vitro shoot organogenesis of Aloe vera (L.) Burm. f | |
