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dc.creatorZielińska,Krystyna
dc.creatorFabiszewska,Agata
dc.creatorStefańska,Ilona
dc.date2015-09-01
dc.date.accessioned2019-04-24T21:20:08Z
dc.date.available2019-04-24T21:20:08Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-58392015000400005
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/56063
dc.descriptionThere is a significant range of bacterial inoculants for forage ensiling, but there is still a need for formulations to improve the safety of feed. The objective of the study was to evaluate the usefulness of three lactobacilli strains in alfalfa (Medicago sativa L.) The following bacterial inoculants were used: Lactocacillus plantarum K KKP 593p (LPK), L. plantarum C KKP 788p (LPC), L. buchneri KKP 907p (LB), and mix of all three strains (LPK+LPC+LB). The application of bacterial inoculants in alfalfa ensiling resulted in a reduction of the total number of molds, Clostridium perfringens and Listeria sp. (up to 5, 7, and 5 times respectively for LB inoculant in comparison to untreated silage). Total inhibition of Salmonella sp. and Escherichia coli growth was achieved in silages treated with all inoculants except for LPC. Aerobic stability in the control silage was the lowest (77 h) and doubled under the influence of bacterial inoculants. The most stable according to aerobic stability was silage treated with LB inoculant (175 h), where the highest concentrations of acetic acid (4.8 g kg-1), propionic acid (0.7 g kg-1) and 1,2-propanediol (526 mg kg-1) were reported. The study discussed that it is important to evaluate not only the effect of bacterial inoculants on physicochemical and microbiological silage properties, as the presence and expression of antibiotic resistance genes in lactic acid bacteria have been reported. The results of antimicrobial susceptibility testing of the strains showed that almost all minimum inhibitory concentrations values for eight antibiotics were equal to or below the corresponding breakpoints proposed by the European Food Safety Authority, Additives and Products or Substances used in Animal Feed Panel.
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dc.languageen
dc.publisherInstituto de Investigaciones Agropecuarias, INIA
dc.relation10.4067/S0718-58392015000400005
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceChilean journal of agricultural research v.75 n.3 2015
dc.subjectAerobic stability
dc.subjectalfalfa
dc.subjectantibiotic susceptibility
dc.subjectLactobacillus
dc.subjectpathogenic bacteria
dc.subjectsilage
dc.titleDifferent aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage


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