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dc.creatorLi,Wei
dc.creatorZhang,Lele
dc.creatorZhao,Ning
dc.creatorGuo,Wenlei
dc.creatorLiu,Weitang
dc.creatorLi,Lingxu
dc.creatorWang,Jinxin
dc.date2017-09-01
dc.date.accessioned2019-04-24T21:20:29Z
dc.date.available2019-04-24T21:20:29Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-58392017000300257
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/56302
dc.descriptionABSTRACT American slough grass (Beckmannia syzigachne [Steud.] Fernald) is a worldwide weed and is widely distributed in rice-wheat rotations in China. Fenoxaprop-P-ethyl and mesosulfuron-methyl are two major herbicides used to control B. syzigachne. Resistance has evolved in B. syzigachne under continuous selective pressure from herbicides. This study aimed to establish the cross-resistance pattern of a resistant population and explore the potential non-target-site based resistance mechanisms of B. syzigachne. Sequencing of target enzyme genes (acetyl coenzyme A carboxylase [ACCase] and acetolactate synthase [ALS]) revealed that there were no resistance-endowing amino acid substitutions in the resistant B. syzigachne population (RF1) compared with the sensitive population (SF1), and obtained the purified materials. Furthermore, piperonyl butoxide (PBO) and malathion showed synergistic effects with fenoxaprop-P-ethyl and mesosulfuron-methyl respectively in RF1. Therefore, we speculate that the resistance observed in B. syzigachne was related with metabolic, mostly involving the cytochrome P450 enzymes. Cross resistance patterns showed that the purified resistant B. syzigachne produce high resistance to fenoxaprop-P-ethyl and pyroxsulam; intermediate resistance to flucarbazone-sodium; low resistance to quizalofop-P-ethyl, clodinafop-propargyl, sethoxydim and mesosulfuron-methyl; sensitive to clethodim pinoxaden and isoproturon.
dc.formattext/html
dc.languageen
dc.publisherInstituto de Investigaciones Agropecuarias, INIA
dc.relation10.4067/S0718-58392017000300257
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceChilean journal of agricultural research v.77 n.3 2017
dc.subjectHerbicide resistance
dc.subjectmetabolic resistance
dc.subjectP450 inhibitors
dc.subjecttarget enzymes sequencing.
dc.titleMultiple resistance to ACCase and ALS-inhibiting herbicides in Beckmannia syzigachne (Steud.) Fernald without mutations in the target enzymes


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