ABSTRACT Agronomy practices aimed at wheat (Triticum aestivum L.) grain Zn biofortification are important to alleviate Zn deficiency in humans, especially for those resource-poor people owing to subsistence on diets dominated by cereal-based foods with low concentrations and bioavailability of Zn. To promote understanding of source-sink flow processes affecting biofortification of wheat with Zn, effects of different source/sink manipulations on grain Zn accumulation by wheat were examined in pot and field at two locations. Treatments included foliar applications of deionized water, sucrose, ZnSO4, sucrose + Zn, defoliation, spike shading or partial removal of spikes. Results showed sucrose + Zn significantly increased grain Zn concentration more than Zn alone. Grain Zn concentration with sucrose + Zn averaged 66 mg kg-1 for &#8216;Kenong 9204&#8217; and 59 mg kg-1 for &#8216;Liangxing 99&#8217; in pot, and ranged from 42 to 58 mg kg-1 for &#8216;Liangxing 99&#8217;, &#8216;Jinan 17&#8217;, &#8216;Jimai 20&#8217;, &#8216;Jimai 22&#8217;, and &#8216;Luyuan 502&#8217; under field, approaching the field-grown target 60 mg kg-1 proposed by World Health Organization. Molar ratios of phytic acid/Zn and phytic acid × Ca/Zn of &#8216;Jinan 17&#8217;, &#8216;Jimai 20&#8217; or &#8216;Luyuan 502&#8217; with sucrose + Zn were reduced to be < 15 and < 200, respectively, suggesting higher Zn bioavailability. Defoliation or spike shading decreased grain weight, Zn concentration and content. It is concluded that grain Zn accumulation of wheat can be affected by the source-sink relationship of Zn and/or carbohydrate, and the foliar spray &#8216;sucrose + Zn&#8217; is recommended for increasing concentration and bioavailability of Zn in wheat grains.