ABSTRACT: 31. Objective: To analyze differences in health, leisure and work accord ing to work shift in industry workers of Florianopolis, Brazil. Method: participants were 885 workers on fixed work shift (649 of the gen eral shift, 101 of the 1st shift, 84 of the 2nd shift and 51 of the 3rd shift). Health was investigated using the International Physical Activity Questionnaire - Short Form (IPAQ-SF), the Pittsburgh Sleep Quality Index (PSQI) and the Horne-Ostberg morningness and eve ningness questionnaire for physical activity, sleep quality and chro- notype, respectively. Leisure activities were investigated based on the Scale of practices in leisure (Escala de Práticas no Lazer - EPL). To calculate difference between means, the Kruskall-Wallis test (Dunn or Student-Newman-Keuls post hoc) and the chi-square test were used. In the correlation analysis among continuous variables, the Spearman test was used. Data were analyzed using SPSS 20.0, BioEstat 5.0 and GPower 3.0 softwares. A 5% significance level was adopted. Results: The average age of workers was 31.1 (8.5) years, and 58.0% were female. Significant differences among work shifts with respect to sleep quality (p<0.001) were identified, with prevalence of 35.6% of sleep disorders in the night shift. Among these, 16.7% showed morn ing characteristics and 68.6% reported absence from work for health reasons in the last year. The consumption of caffeinated beverages (33.3%) and cigarettes (21.6%) were also higher among night shift workers. Nevertheless, the time in vigorous physical activity was higher, averaging 75.5 minutes per week. Furthermore, night shift workers had lower income (p<0.001) and seek a second job for income complementation (p=0.002). Among issues related to leisure, differences in artistic (p=0.046), physical-sport (p=0.048) and touris tic content (p=0.004) were identified. Conclusion: substantial differ ences regarding labor, health and leisure issues were evidenced in different shifts, especially in the night shift. Although, theoretically, there is more time for leisure activities, night shift workers seek for a second job for income complementation. The excess of work associ ated with waking at night and risk behaviors result in damages to health and leisure.