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dc.creatorRadhamony,Resmi Nath
dc.creatorMohan Prasad,Anand
dc.creatorSrinivasan,Ramamurthy
dc.date2005-04-01
dc.date.accessioned2019-05-03T12:44:11Z
dc.date.available2019-05-03T12:44:11Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100012
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/84813
dc.descriptionWith the availability of complete genome sequences of several organisms, the focus has shifted from structural genomics to functional genomics, specifically in plants where the complete genomic sequences are becoming available i.e., Arabidopsis and rice. Agrobacterium mediated transformation which is exploited for transgenic technology is also being used as an effective mutagen and as a tool for functional genomics in higher plants. Besides the fact that the insertion of T-DNA element into a gene can lead to loss or gain of function, ingenious use of a variety of vectors have led to the identification of genes and regulatory elements in Arabidopsis. In this review, we highlight the progress made in the field of functional genomics of Arabidopsis using T-DNA tagging. Since this strategy has been very successfully employed in Arabidopsis and is now being extended to other plant species, we discuss the various vectors and experimental approaches employed to tag, identify and clone genes and promoter elements in Arabidopsis using T-DNA as a tool.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.relation10.4067/S0717-34582005000100012
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.8 n.1 2005
dc.subjectArabidopsis
dc.subjectfunctional genomics
dc.subjectinsertional mutagenesis
dc.subjectpromoter trapping
dc.subjectT-DNA tagging
dc.titleT-DNA insertional mutagenesis in Arabidopsis: a tool for functional genomics


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