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  • Electronic Journal of Biotechnology
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  • Pontificia Universidad Católica de Valparaíso
  • Electronic Journal of Biotechnology
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Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects

Author
Pavlov,Maria Soledad

Eissler,Yoanna

Espinoza,Juan Carlos

Garland,David E

Kuznar,Juan

Full text
https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100011
Abstract
A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus with SYBR-Green I or with antibodies for specific fluorescence labeling of viral proteins. The type of surface used as mounting support was assayed as well. The results indicated that the most suitable method involves the mounting of the viral-containing suspension on a membrane filter followed by the staining with a monoclonal antibody specific for a viral protein combined with a FITC (fluorescein isothiocyanate)-conjugated secondary antibody.
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Discipline
Artes, Arquitectura y UrbanismoCiencias Agrarias, Forestales y VeterinariasCiencias Exactas y NaturalesCiencias SocialesDerechoEconomía y AdministraciónFilosofía y HumanidadesIngenieríaMedicinaMultidisciplinarias
Institutions
Universidad de ChileUniversidad Católica de ChileUniversidad de Santiago de ChileUniversidad de ConcepciónUniversidad Austral de ChileUniversidad Católica de ValparaísoUniversidad del Bio BioUniversidad de ValparaísoUniversidad Católica del Nortemore

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