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dc.creatorPavlov,Maria Soledad
dc.creatorEissler,Yoanna
dc.creatorEspinoza,Juan Carlos
dc.creatorGarland,David E
dc.creatorKuznar,Juan
dc.date2011-01-01
dc.date.accessioned2019-05-03T12:44:49Z
dc.date.available2019-05-03T12:44:49Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100011
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85188
dc.descriptionA method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus with SYBR-Green I or with antibodies for specific fluorescence labeling of viral proteins. The type of surface used as mounting support was assayed as well. The results indicated that the most suitable method involves the mounting of the viral-containing suspension on a membrane filter followed by the staining with a monoclonal antibody specific for a viral protein combined with a FITC (fluorescein isothiocyanate)-conjugated secondary antibody.
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dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.14 n.1 2011
dc.subjectdigital image analysis
dc.subjectfluorescence microscope methods
dc.subjectimmunofluorescence
dc.subjectInfectious pancreatic necrosis virus (IPNV)
dc.subjectSYBR-Green I
dc.titleInfectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects


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