| dc.creator | Regalado,Carlos | |
| dc.creator | Vázquez-Obregón,Immer | |
| dc.creator | García-Almendárez,Blanca Estela | |
| dc.creator | Domínguez-Domínguez,Jorge | |
| dc.creator | Aguilera-Barreyro,Araceli | |
| dc.creator | Amaro-Reyes,Aldo | |
| dc.date | 2011-03-01 | |
| dc.date.accessioned | 2019-05-03T12:44:50Z | |
| dc.date.available | 2019-05-03T12:44:50Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200009 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85198 | |
| dc.description | Hemicellulosic agricultural by-products such as corn stover (CS) are highly available materials which represent an opportunity to develop value added products. Native Aspergillus niger GS1 was used for solid-state fermentation (SSF) on alkali pre-treated CS (ACS) aimed to optimize xylanolytic enzymes production, and their effect on in vitro ruminal and true digestibility of ACS. Enzyme production was empirically modelled using a fractional factorial design 2(9-5), and the resulting significant factors were glucose, yeast extract and two mineral salts, which were arranged in a Draper-Lin optimization design at two levels. Predicted optimum xylanolytic activity of 33.6 U (mg protein)-1 was achieved at 48 hrs of SSF, and was validated by confirmatory experiments. ACS was incubated with a semipurified enzymatic extract (EE) showing a xylanolytic activity of 1600 U kg-1 dry ACS for 12 hrs before exposure to cow?s ruminal liquid for 72 hrs, which led to 5% and 10% increase of in vitro ruminal and true digestibility, respectively. CS is a readily available by-product in different regions which after alkaline treatment and partial hydrolysis with the EE, may be advantageously used as supplement for ruminant feed. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.14 n.2 2011 | |
| dc.subject | Aspergillus niger GS1 | |
| dc.subject | corn stover | |
| dc.subject | digestibility | |
| dc.subject | solid-state fermentation | |
| dc.subject | xylanolytic activity | |
| dc.title | Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility | |
