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dc.creatorRegalado,Carlos
dc.creatorVázquez-Obregón,Immer
dc.creatorGarcía-Almendárez,Blanca Estela
dc.creatorDomínguez-Domínguez,Jorge
dc.creatorAguilera-Barreyro,Araceli
dc.creatorAmaro-Reyes,Aldo
dc.date2011-03-01
dc.date.accessioned2019-05-03T12:44:50Z
dc.date.available2019-05-03T12:44:50Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200009
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85198
dc.descriptionHemicellulosic agricultural by-products such as corn stover (CS) are highly available materials which represent an opportunity to develop value added products. Native Aspergillus niger GS1 was used for solid-state fermentation (SSF) on alkali pre-treated CS (ACS) aimed to optimize xylanolytic enzymes production, and their effect on in vitro ruminal and true digestibility of ACS. Enzyme production was empirically modelled using a fractional factorial design 2(9-5), and the resulting significant factors were glucose, yeast extract and two mineral salts, which were arranged in a Draper-Lin optimization design at two levels. Predicted optimum xylanolytic activity of 33.6 U (mg protein)-1 was achieved at 48 hrs of SSF, and was validated by confirmatory experiments. ACS was incubated with a semipurified enzymatic extract (EE) showing a xylanolytic activity of 1600 U kg-1 dry ACS for 12 hrs before exposure to cow?s ruminal liquid for 72 hrs, which led to 5% and 10% increase of in vitro ruminal and true digestibility, respectively. CS is a readily available by-product in different regions which after alkaline treatment and partial hydrolysis with the EE, may be advantageously used as supplement for ruminant feed.
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dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.14 n.2 2011
dc.subjectAspergillus niger GS1
dc.subjectcorn stover
dc.subjectdigestibility
dc.subjectsolid-state fermentation
dc.subjectxylanolytic activity
dc.titleXylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility


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