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dc.creatorWang,Chuan Tang
dc.creatorTang,Yue Yi
dc.creatorWang,Xiu Zhen
dc.creatorZhang,Shu Wei
dc.creatorLi,Gui Jie
dc.creatorZhang,Jian Cheng
dc.creatorYu,Shan Lin
dc.date2011-03-01
dc.date.accessioned2019-05-03T12:44:50Z
dc.date.available2019-05-03T12:44:50Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000200010
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/85199
dc.descriptionScreening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with >60% oleate thus identified were further confirmed by gas chromatography. Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild type) and peanut seeds with elevated oleate (mutant type) revealed a C281T transition in the coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the enzyme activity.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceElectronic Journal of Biotechnology v.14 n.2 2011
dc.subjectchemical mutant
dc.subjectFAD2B
dc.subjectNIRS
dc.subjectoleate
dc.subjectpeanut
dc.titleSodium azide mutagenesis resulted in a peanut plant with elevated oleate content


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