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  • Electronic Journal of Biotechnology
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  • Pontificia Universidad Católica de Valparaíso
  • Electronic Journal of Biotechnology
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Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.)

Author
Tang,Yue Yi

Wang,Chuan Tang

Yang,Guan Pin

Feng,Tong

Gao,Hua Yuan

Wang,Xiu Zhen

Chi,Xiao Yuan

Xu,Ya Long

Wu,Qi

Chen,Dian Xu

Full text
https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500005
Abstract
To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR).
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Discipline
Artes, Arquitectura y UrbanismoCiencias Agrarias, Forestales y VeterinariasCiencias Exactas y NaturalesCiencias SocialesDerechoEconomía y AdministraciónFilosofía y HumanidadesIngenieríaMedicinaMultidisciplinarias
Institutions
Universidad de ChileUniversidad Católica de ChileUniversidad de Santiago de ChileUniversidad de ConcepciónUniversidad Austral de ChileUniversidad Católica de ValparaísoUniversidad del Bio BioUniversidad de ValparaísoUniversidad Católica del Nortemore

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