| dc.creator | Tang,Yue Yi | |
| dc.creator | Wang,Chuan Tang | |
| dc.creator | Yang,Guan Pin | |
| dc.creator | Feng,Tong | |
| dc.creator | Gao,Hua Yuan | |
| dc.creator | Wang,Xiu Zhen | |
| dc.creator | Chi,Xiao Yuan | |
| dc.creator | Xu,Ya Long | |
| dc.creator | Wu,Qi | |
| dc.creator | Chen,Dian Xu | |
| dc.date | 2011-09-01 | |
| dc.date.accessioned | 2019-05-03T12:44:53Z | |
| dc.date.available | 2019-05-03T12:44:53Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500005 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/85231 | |
| dc.description | To isolate differentially expressed peanut genes responsive to chilling, a suppression subtractive hybridization (SSH) cDNA library was constructed for a chilling tolerant peanut cultivar A4 with mRNAs extracted from the seeds imbibed at 2ºC and 15ºC, respectively, for 24 hrs. A total of 466 cDNA clones were sequenced, from which 193 unique transcripts (73 contigs and 120 singlets) were assembled. Of these unique transcripts, 132 (68.4%) were significantly similar to the sequences in GenBank non-redundant (nr) protein database, which belonged to diverse functional categories including metabolism, signal transduction, stress response, cell defense and transcriptional regulation. The remaining 61 (31.6%) showed no similarity to either hypothetical or known proteins. Six differentially expressed transcripts were further confirmed with real-time quantitative PCR (RT-qPCR). | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Electronic Journal of Biotechnology v.14 n.5 2011 | |
| dc.subject | chilling response | |
| dc.subject | peanut | |
| dc.subject | real-time quantitative PCR | |
| dc.subject | suppression subtractive hybridization | |
| dc.title | Identification of chilling-responsive transcripts in peanut (Arachis hypogaea L.) | |
