In cultivated aquatic organisms nutritional requirements are critical, not only for their impact on production techniques, but also, for their high incidence on production costs. There is limited knowledge on some species such as the southern Chilean freshwater crayfish, Samastacus spinifrons. In order to generate practical knowledge, a study was carried out to determine protein and carbohydrate content requirements. These factors were evaluated upon their effects on growth and survival of juveniles. For this purpose, individual weight, biomass gain, survival, and feed conversion parameters were measured. The assay was carried out in 42 days, it was conducted in a flow through system, using 21 plastic tanks of 10.6 L capacity. Each tank was seeded with 20 juveniles weighing 50 mg average each. A 3×2 factorial design was proposed with three protein contents (20, 30, 40%) and two carbohydrate contents (low: from 16.3 to 23.5% and high: from 34.6 to 35.8%). Six treatments and three replicates were performed. Individuals were fed on apparent satiation once a day. The diets formulated with 30% of protein and the two carbohydrate contents resulted in higher biomass increases, food conversion efficiencies over 26%, and specific growth rate of 0.78%, all displaying significant differences. Survival showed highly significant differences; in all diets were superior to 60%, however the diets with 30% of protein surpassed 90%.