| dc.creator | García,Silvano | |
| dc.creator | Yasui,George S | |
| dc.creator | Bernardes-Júnior,Jurandir J | |
| dc.creator | Corrêa da Silva,Bruno | |
| dc.creator | Amaral-Júnior,Hilton | |
| dc.creator | Zaniboni-Filho,Evoy | |
| dc.date | 2017-03-01 | |
| dc.date.accessioned | 2019-05-03T13:28:07Z | |
| dc.date.available | 2019-05-03T13:28:07Z | |
| dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-560X2017000100022 | |
| dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/88298 | |
| dc.description | This study evaluated sequential double-temperature shock (heat and cold) to induce triploidy in Rhamdia quelen. Fertilized eggs were heat shocked at 37, 39 and 41°C at 2 min post-fertilization and then cold shocked at 1°C for 20 min. Intact eggs were used as controls. Egg viability in control groups was higher (65.5 ± 37.0%) than that at 37°C (58.2 ± 37.7%), 39°C (1.8 ± 0.3%) and 41°C (0%). The survival of control hatchery (64.6 ± 36.8%) was higher compared to treatments at 37°C (24.4 ± 15.5%) and 39°C (0.6 ± 0.07%). Triploid percentages were 98.5% at 37°C and 100% at 39°C. Therefore, while double-temperature shock induced 100% triploidy in R. quelen, it also significantly decreased embryo survival and increased the deformity larvae. | |
| dc.format | text/html | |
| dc.language | en | |
| dc.publisher | Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar | |
| dc.relation | 10.3856/vol45-issue1-fulltext-22 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.source | Latin american journal of aquatic research v.45 n.1 2017 | |
| dc.subject | Rhamdia quelen | |
| dc.subject | silver catfish | |
| dc.subject | polyploidy | |
| dc.subject | temperature shock | |
| dc.subject | flow cytometry | |
| dc.title | Induction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shock | |
