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dc.creatorOrtiz Tirado,Juan
dc.creatorValladares,Luis
dc.creatorMuñoz,Daysi
dc.creatorCaza,Jairo
dc.creatorManjunatha,Bangeppagari
dc.creatorR. Kundapur,Rajesh
dc.date2017-11-01
dc.date.accessioned2019-05-03T13:28:14Z
dc.date.available2019-05-03T13:28:14Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-560X2017000500930
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/88371
dc.descriptionABSTRACT Circulating levels of estradiol, vitellogenin and prostaglandins could be references of oocytes quality in the aquaculture industry. This study evaluated the performance of 17&#946;-estradiol (E2) and vitellogenin (Vtg) levels in plasma and prostaglandins in oocytes (PGE 2) during the reproductive period of Oreochromis niloticus in a commercial farm in Ecuador. Adults fish females were treated with estradiol-synthetic (ES) and tilapia pituitary extracts (TP) during 25 days. For quantification of E2 and PGE 2, an enzyme-linked immunosorbent assay (ELISA) was used, while for Vtg relative quantification a Western blot analysis was performed. Circulating E2 concentrations were different during the first week of follow-up (P < 0.05) resulting in different levels of Vtg per treatment (P < 0.05) affecting on gonadal growth. The ovaries had an asynchronous development, where the mature oocytes proportions were higher with ES and TP than control. Relative fecundity was different between treatments (P < 0.05), where TP generated 15.7 ± 5.8 oocytes per g of spawning female. Furthermore, concentrations of PGE 2 were different between treatments (P < 0.05). The information reported in this study will be useful to improve the reproduction methods and have some biological signals before ovulation in tilapia commercial culture.
dc.formattext/html
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
dc.relation10.3856/vol45-issue5-fulltext-8
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceLatin american journal of aquatic research v.45 n.5 2017
dc.subjectOreochromis niloticus
dc.subjecttilapia
dc.subjectestradiol
dc.subjectvitellogenin
dc.subjectpituitary
dc.subjectprostaglandin E2
dc.titleLevels of 17&#946;-estradiol, vitellogenin, and prostaglandins during the reproductive cycle of Oreochromis niloticus


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