dc.creator | Ortiz Tirado,Juan | |
dc.creator | Valladares,Luis | |
dc.creator | Muñoz,Daysi | |
dc.creator | Caza,Jairo | |
dc.creator | Manjunatha,Bangeppagari | |
dc.creator | R. Kundapur,Rajesh | |
dc.date | 2017-11-01 | |
dc.date.accessioned | 2019-05-03T13:28:14Z | |
dc.date.available | 2019-05-03T13:28:14Z | |
dc.identifier | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-560X2017000500930 | |
dc.identifier.uri | http://revistaschilenas.uchile.cl/handle/2250/88371 | |
dc.description | ABSTRACT Circulating levels of estradiol, vitellogenin and prostaglandins could be references of oocytes quality in the aquaculture industry. This study evaluated the performance of 17β-estradiol (E2) and vitellogenin (Vtg) levels in plasma and prostaglandins in oocytes (PGE 2) during the reproductive period of Oreochromis niloticus in a commercial farm in Ecuador. Adults fish females were treated with estradiol-synthetic (ES) and tilapia pituitary extracts (TP) during 25 days. For quantification of E2 and PGE 2, an enzyme-linked immunosorbent assay (ELISA) was used, while for Vtg relative quantification a Western blot analysis was performed. Circulating E2 concentrations were different during the first week of follow-up (P < 0.05) resulting in different levels of Vtg per treatment (P < 0.05) affecting on gonadal growth. The ovaries had an asynchronous development, where the mature oocytes proportions were higher with ES and TP than control. Relative fecundity was different between treatments (P < 0.05), where TP generated 15.7 ± 5.8 oocytes per g of spawning female. Furthermore, concentrations of PGE 2 were different between treatments (P < 0.05). The information reported in this study will be useful to improve the reproduction methods and have some biological signals before ovulation in tilapia commercial culture. | |
dc.format | text/html | |
dc.language | en | |
dc.publisher | Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar | |
dc.relation | 10.3856/vol45-issue5-fulltext-8 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.source | Latin american journal of aquatic research v.45 n.5 2017 | |
dc.subject | Oreochromis niloticus | |
dc.subject | tilapia | |
dc.subject | estradiol | |
dc.subject | vitellogenin | |
dc.subject | pituitary | |
dc.subject | prostaglandin E2 | |
dc.title | Levels of 17β-estradiol, vitellogenin, and prostaglandins during the reproductive cycle of Oreochromis niloticus | |