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dc.creatorManríquez-Santos,Teresa de Jesús
dc.creatorÁlvarez-González,Carlos Alfonso
dc.creatorPeña,Emyr
dc.creatorCamarillo-Coop,Susana
dc.creatorMartínez-García,Rafael
dc.creatorVega-Villasante,Fernando
dc.date2018-07-01
dc.date.accessioned2019-05-03T13:28:21Z
dc.date.available2019-05-03T13:28:21Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0718-560X2018000300525
dc.identifier.urihttp://revistaschilenas.uchile.cl/handle/2250/88443
dc.descriptionABSTRACT The present research was focused to characterize the digestive proteases in Macrobrachium carcinus adults using biochemical and electrophoretic techniques. Our results showed that the alkaline proteolytic activity from males and females did not show significant differences (P > 0.05) between them, the optimum pH for digestive proteases is 8, and is very stable to changes in alkaline pH (8 and 10). The optimum temperature for alkaline proteases is 45°C and is stable from 25 to 45°C. The activity was totally inhibited with phenylmethylsulfonyl fluoride (PMSF), additionally the inhibition with trypsin soybean inhibitor 1 (SBT1) and tosyl-lysine-methyl ketone (TLCK) indicate high effect over serine proteases. Eight active bands were found using sodium dodecyl sulfate polyacrylamide electrophoresis (SDS-PAGE) zymogram (range 17.8-94.0 kDa), which were partially inhibited with ovalbumin (Ovo), SBT1, phenanthroline (Phen), tosyl-phenylalanine-methyl ketone (TPCK), TLCK and ethylenediaminetetraacetic acid (EDTA), indicating an omnivorous digestive capacity, which remarks that the mainly alkaline protease in M. carcinus hepatopancreas are trypsin like enzymes.
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dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
dc.relation10.3856/vol46-issue3-fulltext-5
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceLatin american journal of aquatic research v.46 n.3 2018
dc.subjectMacrobrachium carcinus
dc.subjectenzymes
dc.subjectnutrition
dc.subjectproteases
dc.subjectinhibitors
dc.titlePartial characterization of digestive proteases in adults of bigclaw river shrimp Macrobrachium carcinus


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