ABSTRACT Biofloc system is an alternative to conventional aquaculture systems as it minimizes effluents, improves water quality, ensures greater biosafety due to minimal water exchanges and also serves as an additional food source for prawn. The present study aimed to evaluate the effect of suppression of vitamin and mineral supplements on diets for Macrobrachium rosenbergii reared in the biofloc system. Four experimental diets were evaluated: complete diet (Diet 1 with vitamin and mineral supplementation); diet without vitamin supplement (Diet 2); diet without mineral supplement (Diet 3) and diet without vitamin and mineral supplement (Diet 4). The experimental design was completely randomized with four replicates per treatment. After 45 days of trial survival rates above 90% and feed conversion rate ~1.83 were observed for all treatments, indicating that the production of M. rosenbergii in the biofloc system does not require the inclusion of vitamin and mineral supplementation in the feed.