ABSTRACT In the present study, the potential of three isolated microalgae strains from Bahía de La Paz, Baja California Sur, Mexico and identified as Schizochytrium sp. (Inner key: LPU-1), Chaetoceros sp. (LPU-2), and Chaetoceros sp. (LPU-3) for the cultivation of the rotifer Brachionus plicatilis was tested. The effects of isolated strains on the population growth and nutritional content (proteins, lipids, carbohydrates, and fatty acid composition) of B. plicatilis were evaluated. The feeding essay of B. plicatilis was carried out at 32 ± 2°C. Treatments were established using a monoalgal and dialgal diet: Schizochytrium sp. (LPU-1), Schizochytrium sp. (LPU-1)/Chaetoceros sp. (LPU-2), Chaetoceros sp. (LPU-2), Chaetoceros sp. (LPU-3), Chaetoceros sp. (LPU-2)/Chaetoceros sp. (LPU-3). The results show that the rotifers population growth ratio was higher with Schizochytrium sp. (LPU-1) 0.88 ± 0.43, Chaetoceros sp. (LPU-3), 0.87 ± 0.37, and from both 0.87 ± 0.40. The results show that the native microalgae of a North Pacific area mixed are an excellent source of nutrients for the growth and enhancement of the nutritional value of the rotifers, which can be used in the future to feed the larvae of marine fish more nutritionally and economically.