Two wheat fertilization experiments were conducted during the 2005-2006 and 2007-2008 seasons using a medial, mesic, seric, Typic Placandept soil to determine the effect of sulfur on the yield, percentage of protein, gluten content, and sedimentation volume of the grain and the nutrient availability in the soil. During the 2005-06 season, the fertilization treatments consisted of a base fertilization plus various sulfur treatments: 0 kg S ha-1 (T1); 27 kg S ha-1 (T2); 54 kg S ha-1 (T3) or 108 kg S ha-1 (T4). During the 2007-08 season, the fertilization treatments were 0 kg S ha-1 (T1), 9 kg S ha-1 (T2), 18 kg S ha-1 and 36 kg S ha-1 (T4). The results showed no differences (P<0.05) in the grain yield for any of the treatments evaluated. Furthermore, no increases were found for any of the industrial quality variables analyzed. In contrast, increases in the availability of sulfur in the soil were observed. Sulfur application in soils having contents of 12 and 13 mg kg-1 S did not increase the grain yield, protein content, gluten, or sedimentation volume.